ABSTRACT
Erythrasma is a chronic superficial infection of the intertriginous areas. Most laboratories use methylene blue stain and 10% KOH smear to identify Corynebacterium minutissimum [C. minutissimum] by direct observation of filamentous bacilli. Occasionally atypical forms can be seen that create problems in diagnosis. This study aims to use the polymerase chain reaction [PCR] method in order to definitively identify C. minutissimum as an agent of erythrasma. This research was performed during 2013 on 100 skin scrapings suspicious for erythrasma which were obtained from various medical mycology laboratories in Tehran. Samples were tested by three methods - direct examination, culture and PCR. DNA was extracted by the modified phenol-chloroform method after which PCR was performed using designed primers. We sequenced some of the PCR products. The sensitivity and specificity of the PCR method was compared to the direct and culture examinations. Of the 100 samples, there were 25 positive samples according to PCR analysis, 13 positive by direct examination and 23 that cultured positive. DNA sequencing results showed the presence of C. minutissimum. The PCR method in comparison with direct examination had a sensitivity of 100% and a specificity of 86.2%. The study also showed that the PCR method in comparison with culture had a sensitivity of 100% and a specificity of 97.4%. This study showed that the PCR method in comparison with the direct method and culture had a higher sensitivity in the detection of C. minutissimum. The present PCR method confirmed all typical and some of the atypical forms of C. minutissimum which indicated the importance of this method in the definitive diagnosis of erythrasma
Subject(s)
Humans , Erythrasma/diagnosis , Corynebacterium/genetics , Skin/pathology , Corynebacterium/isolation & purification , Polymerase Chain ReactionABSTRACT
El eritrasma es una infección superficial de la piel que afecta la capa córnea y es causado por una bacteria grampositiva, Corynebacterium minutissimum. Se presenta como manchas eritematosas a marrón en grandes pliegues y como descamación y maceración en pliegues interdigitales; es asintomática, aunque en algunos casos se acompaña de prurito. El diagnóstico es clínico por luz de Wood que da una fluorescencia rojo coral de las áreas afectadas. Su curso es benigno, aunque persiste si no se da un tratamiento adecuado.
Erythrasma is a superficial skin infection of the stratum corneum caused by a gram-positive bacteria, Corynebacterium minutissimum. It is characterized by reddish-brown areas affecting occluded intertriginous zones such as axillae, inframammary folds, and as irregular scaly patches or macerated lesions on toeweb spaces. It is asymptomatic, though sometimes accompanied by pruritus. Clinical diagnosis is supported by a red fluorescence under Wood s light examination. It is benign, yet persistent if not adequately treated.
Subject(s)
Humans , Erythrasma/diagnosis , Erythrasma/therapy , Diagnosis, Differential , Erythrasma/epidemiology , Erythrasma/microbiology , Corynebacterium Infections/complications , PrognosisABSTRACT
El eritrasma es una enfermedad asintomática que pertenece a las corinebacteriosis. En un estudio retrospectivo se encontraron cinco casos de eritrasma en grandes pliegues en 30 meses. Se presenta un estudio prospectivo de cuatro casos de eritrasma de topografía interdigitopodal en un periodo de un año, donde la lámpara de Wood demuestra su utilidad diagnóstica. La frecuencia relativa aumentó de 2.7 a 4.7 por 1,000
Subject(s)
Humans , Male , Female , Adult , Middle Aged , Corynebacterium , Corynebacterium Infections , Corynebacterium Infections/diagnosis , Erythrasma , Erythrasma/diagnosisABSTRACT
Se comentan los datos históricos, microbiológicos, patógenos y epidemiológicos del eritrasma, así como sus características clínicas y de laboratorio y su tratamiento. El agente causal es Corynebacterium minutissimum y se menciona que el eritrasma es una enfermedad común en nuestro medio, aunque se desconoce su frecuencia real. Es posible que sea mal diagnosticada o poco reportada debido a su relativamente fácil diagnóstico o a su favorable reacción ante los diversos tratamientos existentes
Subject(s)
Humans , Corynebacterium Infections , Corynebacterium Infections/diagnosis , Corynebacterium/pathogenicity , Erythrasma , Erythrasma/diagnosis , Erythrasma/drug therapy , Erythrasma/history , Erythrasma/microbiology , Ultraviolet RaysABSTRACT
E descrito um metodo simples e pratico para corar Malassezia furfur e Corynebacterium minutissimum nas escamas das lesoes. O material e colhido com o auxilio de fita durex que sera usada na maior parte das etapas do metodo para ajudar a facil execucao do processo de coloracao. Para colher as escamas, pequenos pedacos de fita durex com cerca de 4 cm de comprimento por 2 cm de largura sao colocados e pressionados sobre as lesoes, e quando retirados trazem aderidas as escamas furfuraceas na face com goma. Esses pedacos de fita durex sao imersos por alguns minutos no corante lactofenol-azul cotton e logo que as escamas estiverem coradas em azul sao lavadas em agua corrente para remover o excesso de corante azul, secos com papel de filtro, desidratados pela passagem em dois frascos de centrifugacao com xilol. O xilol dissolve a goma da fita durex e as escamas caem soltas no tubo. Apos centrifugacao e decantacao as escamas concentradas no fundo do tubo sao colhidas com alca de platina, colocadas em balsamo de Canada sobre uma lamina de microscopia e fechadas com uma laminula...
Subject(s)
Staining and Labeling/methods , Corynebacterium/classification , Erythrasma/diagnosis , Corynebacterium/ultrastructure , MicroscopyABSTRACT
La tinción del PAS más DMSO, es un método sencillo y rápido mediante el cual las raspaduras de lesiones de la piel o de la uñas, recogidas con una laminilla a la que se le ha aplicado albúmina de Mayer en su superficie, puede examinarse microscópicamente y determinar con precisión la ausencia o presencia de hongos y de Corynebacterium minutissimum. El método, además, permite obtener preparaciones permanentes